大鼠心肌细胞(H9c2(2-1)-CAS9)

价 格 : ¥188 ¥288
货 号 : YC-A007-Cas9-H
规 格 : 1*10^6
形 态 : 呼吸道细胞

This is product descrition, HuH-7 fluorescent protein expression cell line, which can stably and efficiently express EGFP fluorescent protein and can be detected by bioluminescence in vitro and in vivo.

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(1) 易于使用:这类细胞系稳定表达Cas9蛋白,仅通过转染gRNA即可实现基因敲除,而通过同时转染gRNA和供体DNA可实现基因敲入/点突变。

(2) 高效敲除:在这类细胞的基础上成功构建了大量基因敲除细胞系,基因敲除效率提高了5-10倍。

(3) 选择的细胞:这种类型的细胞系经过优化,具有低代、高活性和良好的状态,适合于各种基因编辑实验。
Programmable RNA editing enables reversible recoding of RNA information for research and disease treatment. Previously, we developed a programmable A to I RNA editing approach by fusing catalytically inactivated RNA-targeting CRISPR-Cas13 (dCas13) with the adenine deaminase domain of ADAR2. Here, we report a C to U RNA editor, referred to as RNA Editing for Specific C to U Exchange (RESCUE), by directly evolving ADAR2 into a cytidine deaminase. RESCUE doubles the number of pathogenic mutations targetable by RNA editing and enables modulation of phospho-signaling-relevant residues. We apply RESCUE to drive β-catenin activation and cellular growth. Furthermore, RESCUE retains A to I editing activity, enabling multiplexed C to U and A to I editing through the use of tailored guide RNAs.