This is product descrition, HuH-7 fluorescent protein expression cell line, which can stably and efficiently express EGFP fluorescent protein and can be detected by bioluminescence in vitro and in vivo.
ProgrammableRNA editing enables reversible recoding of RNA information for research and disease treatment. Previously, we developed a programmable A to I RNA editing approach by fusing catalytically inactivated RNA-targeting CRISPR-Cas13 (dCas13) with the adenine deaminase domain of ADAR2. Here, we report a C to U RNA editor, referred to as RNA Editing for Specific C to U Exchange (RESCUE), by directly evolving ADAR2 into a cytidine deaminase. RESCUE doubles the number of pathogenic mutations targetable by RNA editing and
enables modulation of phospho-signaling-relevant residues. We apply
RESCUE to drive β-catenin activation and cellular growth. Furthermore,
RESCUE retains A to I editing activity, enabling multiplexed C to U and A to I editing through the use of tailored guide RNAs.